Polymer Surface Modification and Micropatterning for Biomedical and Applications

1 Photografting of Acrylamide onto Poly （ethylene terephthalate)
1.1 Summary
1.2 Introduction
1.3 Materials， reagents and characterization
1.4 Grafting reactor
1.5 Grafting with acrylamide
1.6 Hofmann rearrangement and coupling with fluoro compound
1.7 Characterization of modified surface
1.7.1 Monomer vapour concentration
1.7.2 XPS and contact angle
1.7.3 Coupling reactions
1.8 Conclusions
References
2 Photografting of Maleic Anhydride onto Poly （ethylene terephthalate)
2.1 Summary
2.2 Introduction
2.3 Materials and reagents
2.4 Grafting reactor
2.5 Characterization
2.6 Grafting with maleic anhydride （MAH)
2.7 Conversion of surface functional groups
2.7.1 Hydrolysis of PET-anhydride to PET-COOH
2.7.2 Conversion of carboxylic PET-COOH to acid chloride PET-COCl
2.7.3 Coupling of amino compounds to PET-COC
2.7.4 Coupling of amino compound to PET- anhydride
2.7.5 Coupling of thiol compounds to PET-COOH
2.8 Cell cultivation on PET-RGD surface
2.9 Grafting and characterization
2.10 Coupling reactions
2.11 Surface concentrations of ligands
2.12 Surface grafting effect on cell behaviour
2.13 Conclusion
References
3 Photografting of Acrylamide onto Poly （methyl methacrylate)
3.1 Summary
3.2 Introduction
3.3 Grafting and coupling
3.3.1 Materials and reagents
3.3.2 Grafting reactor
3.3.3 Characterization
3.3.4 Grafting with acrylamide
3.3.5 Hofmann rearrangement
3.3.6 Analysis of amino groups
3.3.7 Coupling with （polyethylene glycol)
3.3.8 Coupling with fluorosubstituted aldehyde
3.4 Characterization of grafted surface
3.4.1 On vapour phase grafting
3.4.2 Characterization using ESCA and contact angle
3.4.3 Functionalization
3.4.4 Coupling reactions
3.5 Conclusions
References
4 Micropatterning Functional Groups on Polymer Surfaces via Masked Vapor-phase Photografting
4.1 Summary
4.2 Introduction
4.3 Preparation of mask and masked grafting
4.3.1 Materials and reagents
4.3.2 Mask preparation and patterned grafting
4.4 On solvent-free vapour-phase photografting
4.5 Micropatterning polymer surface via masked photografting
4.6 Conclusion
References
5 Electron Beam-induced Graft Polymerization of Acrylic Acid and Immobilization of Arginine glycine-aspartic Acidcontaining Peptide onto Nanopatterned Polycaprolactone
5.1 Summary
5.2 Introduction
5.3 Experimental
5.3.1 Material and reagents
5.3.2 Electron beam preirradiation
5.3.3 Graft polymerization
5.3.4 Determination of surface carboxylic groups
5.3.5 Immobilization of RGD peptide
5.3.6 Deactivation of excess disulfide groups
5.3.7 Determination of peptide concentration
5.3.8 Observation of surface topography
5.3.9 Cell cultivation
5.4 Results and discussion
5.4.1 Graft polymerization
5.4.2 Peptide immobilization
5.4.3 Cell behavior
5.5 Conclusions
References
6 Facile Polyester Surface Functionalization via Hydrolysis and Cell-recognizing Peptide Attachment
6.1 Summary
6.2 Introduction
6.3 Experimental
6.3.1 Materials and regents
6.3.2 Preparation of PCL membrane
6.3.3 Hydrolysis of PCL films
6.3.4 Attachment of peptide
6.3.5 Characterization of modified surfaces
6.4 Results and discussion
6.4.1 Preparation of thin films with dip-coating method
6.4.2 Base-catalysed surface hydrolysis
6.4.3 Peptide coupling onto carboxylated surface
6.5 Conclusion
References